Protein aggregation studies: Aggregation studies currently underway involve human serum albumin, lysozyme and the amyloid peptide fragment Ab25-35. Effects of a static electric field on the disruption of fibrils have been reported. Work with polyphenols and surfactants and their effects on the fibrillation process of proteins are in progress. The disruption of the fibrillation of amyloid fibrils, the hallmark of neurodegenerative diseases in the presence of external additives is currently underway.
Studies with eye lens proteins: Modified γ-crystallin (HGCc) from the discarded cataractous emulsion of patients has been isolated and characterized for modifications during cataract formation. Studies showed that the major polyphenol present in green tea, EGCG was able to prevent tryptophan oxidation of the cataractous ocular lens human γ-crystallin in presence of H2O2. The discarded mass is being used to prepare protein nanoparticles and films for applications in drug delivery, wound healing etc. A patent has been awarded for the use of this novel protein source in the preparation of the nanoparticles.
Protein ligand binding: Studies with ribonucleases such as angiogenin, a potent inducer of blood vessel formation, indicated that the process of angiogenesis, the capacity to form blood vessels, was inhibited by polyphenols present in green tea. The polyphenols were also shown to behave as noncompetitive inhibitors of Ribonuclease A. Other inhibitors of the proteins are being developed in collaboration. The effects of oxidative stress on the proteins and methods to combat them are in progress.
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Flavonoid loaded nanoparticles as an effective measure to combat oxidative stress in Ribonuclease A Roy P., Parveen S. , Ghosh P. , Ghatak K. , Dasgupta S. By Biochimie 162 185-197 (2019)
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Positional preferences in flavonoids for inhibition of ribonuclease A: Where -OH where? Tripathy D.R., Panda A., Dinda A.K., Dasgupta S. By Proteins: Structure, Function and Bioinformatics 89 577-587 (2021)
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Tuning the mechanical and physicochemical properties of cross-linked protein films. Parveen S., Chaudhury S. , Dasgupta S. By Biopolymers - (Accepted/In-Press)
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PREPARATION OF NANOPARTICLES DERIVED FROM CATARACTOUS EYE PROTEIN ISOLATE Dasgupta S., Chaudhury S. , Parveen S. By - (2021)
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Design of Configuration-Restricted Triazolylated -DRibofuranosides: A Unique Family of Crescent-Shaped RNase A Inhibitors. Das A., Dasgupta S. , Pathak T. By Org. Biomol. Chem 18 6340-6356 (2020)
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Biodegradable protein films from gallic acid and the cataractous eye protein isolate Parveen S., Chaudhury P., Dasmahapatra U., Dasgupta S. By International Journal of Biological Macromolecules 139 12-20 (2019)
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Effect of Silica Nanoparticles on the Amyloid Fibrillation of Lysozyme Konar M., Mathew A. , Dasgupta S. By ACS Omega 4 1015-1026 (2019)
Principal Investigator
- Glycation of proteins to produce advanced glycation end products and find suitable conditions
- Targeting the Active Site of Aromatase: Chemical Logic Based Design of Potential Anticancer Steroidal and Non-Sreroidal Inhibitors
Co-Principal Investigator
- Centre of excellence for training and research in Microfluidics IIT KHARAGPUR
- Densely Functionalized Divinyl Sulfones- Synthesis Reactions and Applications in Biology and Material Science Council of Scientific and Industrial Research
Ph. D. Students
Atri Sen
Area of Research: Protein Chemistry
B Madhurima
Area of Research: Nano-Bio Science
Goutam Nayak
Area of Research: Protein Chemistry
Kabira Sabnam
Area of Research: Protein Chemistry
Krishna Halder
Area of Research: Protein Chemistry
Krishnananda C P
Area of Research: Nano-Bio Science
Pampa Mondal
Area of Research: Inhibition of ribonucleases with synthetic nucleosides
Piyasi Garai
Area of Research: Computer Simulation Studies of Proteins
Prasun Chowdhury
Area of Research: Protein Chemistry
Sreshtha Chaki
Area of Research: Protein Chemistry
Sujan Santra
Area of Research: Protein Chemistry
Tridib Samanta
Area of Research: Protein Chemistry
